Alyzed distinctive merchandise have been observed. According the mixture was analyzed by NMR; threedifferent solutions have been observed. According toAccording to NMR spectra the crude reaction mixture, trisulfonated, disulfonated and NMR spectra obtained from the crudefrom the crude reaction mixture, trisulfonated, disulfonated NMR spectra obtained from obtained reaction mixture, trisulfonated, disulfonated and monosulfonatedmonosulfonated emodinHowever, the composition in the mixture changed monosulfonatedDMPO medchemexpress emodin had been formed. Having said that, theHowever, the of your mixture changed chan emodin were formed. had been formed. composition composition from the mixture with time and reverse sulfonation occurred. Therefore, work-up procedure, process, with time and reverse sulfonation occurred. Therefore, following thefollowing the work-upthe with time and reverse sulfonation occurred. Hence, following the work-up procedure, crude reaction mixture in stirred in MeCN/hexane 3 the three h was was the crude reaction mixture was stirredMeCN/hexane and soon after afterthehproduct the item was co crude reaction mixture was stirred in was MeCN/hexane and three hand immediately after item totally a stable monosubstituted E_SO3 H, which was which as brown absolutely converted a converted to a steady monosubstituted E_SO3H,isolatedwasaisolated as a bro pletely converted to tostable monosubstituted E_SO3H, which was isolated as a brown solid in 76 strong in 76 yield (Figure three).yield (Figure three). strong in 76 yield (Figure three).Figure three. Synthesis of E_SO3 H. Figure 3. Synthesis of E_SO3H. Figure three. Synthesis of E_SO3H.1,three,8-Trimethoxy-6-methyl-9,10-anthraquinone obtained in obtained in 1,three,8-Trimethoxy-6-methyl-9,10-anthraquinone E_OCH3 was E_OCH3 wasgood yield superior y 1,three,8-Trimethoxy-6-methyl-9,10-anthraquinone E_OCH3 was obtained (MeCO3 )yield (81 ) by applying a previously described reaction process in two 2SO4 and base K2C (81 ) by applying a previously described reaction procedure (Me2 SO4 and base Kgood . (81 ) by applying aBrominated trimethoxyemodin E_Br_OCH3 was 2synthesized from CO3 . previously described reaction process (Me SO4 and base K2 tetrabromina Brominated trimethoxyemodin E_Br_OCH3 was synthesized from tetrabrominated emodin) emodin E_4Br by the E_Br_OCH3 Immediately after purification, the item E_4Br_OCH . Brominated -Irofulven web trimethoxyemodinsame technique. was synthesized from tetrabrominated three was tained in 87 yield. emodin E_4Br by the same approach. Soon after purification, the product E_4Br_OCH3 was obtained in 87 yield. two.2. Antiviral Activity of Emodin AnaloguesThe major objective two.two. Antiviral Activity of Emodin Analogues of this study was to establish the possible of a library o emodin analogues (Figure four) to combat the cytopathic effects of HCoV-NL63. The nMolecules 2021, 26,7 ofE_4Br by the exact same technique. Immediately after purification, the product E_4Br_OCH3 was obtained in 87 yield. 2.2. Antiviral Activity of Emodin Analogues The major objective of this study was to decide the prospective of a library of 8 of 17 analogues (Figure 4) to combat the cytopathic effects of HCoV-NL63. The nontransformed primate kidney Vero cell line was employed for this perform. An further 8 of 17 series Molecules 2021, 26, x FOR PEER Evaluation of research was performed to evaluate the effects of each compound on the viability of Vero cells.Molecules 2021, 26, x FOR PEER Evaluation 11 emodinFigure 4. Emodin and emodin derivatives ready inside the course of this study. Figure four. Emodin and emodin derivatives ready in the c.