The mutants SrtC1Y92A, SrtC2F86A and SrtC1DNT and SrtC2DNT (which include residues 103 of SrtC1 and ninety six of SrtC2, respectively) ended up generated by PIPE mutagenesis [forty one] employing as template the His-MBPSrtC142 and His-MBP- SrtC242 constructs
There are definitely other factors, in vivo, in addition to the LPXTG-like motif, that...