Eagents for DNA synthesis includes Ac-dC-CE Phosphoramidite and the corresponding Ac-dC support, the basis for the UltraFAST cleavage and deprotection system that allows 10 minute deprotection of oligonucleotides. This system requires that the normal benzoyl (Bz) protection of the dC monomer be replaced with acetyl (Ac). The three other monomers remain unchanged.
This seemingly minor change in protecting group leads to oligonucleotides which can be cleaved and deprotected in 10 minutes using AMA which is a 50:50 mixture of aqueous Ammonium hydroxide and aqueous MethylAmine. With AMA the cleavage of the oligonucleotide from the support is accomplished in 5 minutes at room temperature. The deprotection step is carried out at 65 for a further 5 minutes. Deprotection can also be carried out at lower temperatures as follows. In all cases, no base modification has been observed.
MORE NOVEL MONOMERS – EDTA-C2-dT, 7-Deaza-dX, 2′-DEOXYPSEUDOURIDINE
As always, several new phosphoramidites and supports have made a break from our labs. Some never become products but other more enterprising candidates make it into The Glen Report. Here are several new products that we hope will brighten your rsac popcs eerh rset. EDTA-C2-dT About time too! The Dervan group at Caltech described the cleavage of DNA with oligos containing EDTAFe at the C-5 position of Thymidine over 10 years ago.1 Their phosphoramidite contained the triethyl ester of EDTA and our version is shown, (1) in Figure 1. Coupling of EDTA-dT is normal but cleavage and deprotection should be carried out with sodium hydroxide in aqueous methanol (0.4M NaOH in methanol/water 4:1) overnight at room temperature. The cleavage reaction is only i i i t do c F ( I a dd t i t r i o ntae ne eI) n ihohetl are added and so is readily controlled. The Dervan group has described sequence-specific cleavage1 of singlestranded DNA by a probe containing EDTA, as well as cleavage2 of doublestranded DNA following triplex formation by the EDTA-oligo. A later publication described3 the application of t el t e c e v g r a t o t i v s i a e h atr laae ecin o netgt the tertiary structure of RNA.75567-37-2 IUPAC Name 7-Deaza-2′-deoxyXanthosine
(5) 3′-(6-FAM) CPG
As a purine analogue of Thymidine, 7-deaza-2′-deoxyXanthosine (7-deaza-dX) promises to have interesting effects on DNA structure.112809-51-5 supplier Previously, 7-deaza-dX has been shown4 to form stable 7-deaza-X:A-T triplets, allowing triplex formation to occur in the anti-parallel motif.PMID:30860753 7-Deaza-dX has also b e i v s i a e 5 f ri sa i i yt f r a en netgtd o t blt o om non-standard base pair with a 2,4diaminopyrimidine nucleoside analogue. In both cases, 7-deaza-dX was added to the sequence using H-phosphonate chemistry. We offer the phosphoramidite, as shown, (2) in Figure 1, which we have successfully incorporated into oligonucleotides. G
manner analogous to 7-deaza-dG, 7deaza-dX requires the use of (10camphorsulfonyl)oxaziridine (CSO)6 in p a eo t er g l ri d n o i a i ni lc f h eua oie xdto f more than two insertions are to be made into an oligonucleotide. 2′-Deoxypseudouridine Another nucleoside analogue with the potential for affecting DNA structure is the C-nucleoside 2′-deoxypseudouridine. Again this analogue has been i v s i a e 7 f ri sb h v o i t i l netgtd o t eair n rpe helix formation and it has been shown to form stable triplexes with the 2’deoxypseudouridine in the second strand (C:pseudoU-A triplets). Triplex formation was not observed in the same system when U was substituted for pseudoU.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
