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Of Lyon, we decided to prepare an alternative structure that seemed to have a much superior stability profile Methylene Blue II Phosphoramidite (3). Fortunately, this structure did indeed prove more stable and we are now able to offer again a Methylene Blue Phosphoramidite. We recommend a 3 minute coupling for Methylene Blue II Phosphoramidite. In addition, UltraMild monomers and capping must be used to allow deprotection with 0.05M potassium carbonate in methanol (Catalog No. 60-4600-30). The UV/Visible spectrum of an MB labelled oligo is shown in Figure 2. We are happy to provide this unique electrochemical reporter, Methylene Blue II Phosphoramidite, in collaboration with Carole Chaix from the University of Lyon. Methylene Blue II is covered under patent applications FR12 51739 and PCT/FR2013/050356 and is sold under license from the University of Lyon.

Technical Snippets
Question: Why would my synthesis of oligo-dI be disastrous as determined by trityl yield Discussion:
We have never encountered someone who has had problems with the synthesis of oligo-dI. How fast did you see the trityl drop Was it stepwise from the beginning (x % drop every cycle for instance) or did you see the first few trityls looking just fine and then see a quick drop to nothing How many bases did you add in the synthesis before you saw the issue “The trityl was a slow decrease until maybe around base 12-15. We had good color for probably the first 10 or so, with most of the color change happening between bases 10-15. At that point most everything was clear.” We have seen some bases that are sensitive to successive cycles do much better with a low water oxidizer. We suggest trying one more time using the dI you have on the machine and 0.349085-38-7 Biological Activity 5M CSO instead of the standard Iodine based oxidizer (with a 3 minute oxidation time).168555-66-6 web “We were able to run the poly dI with CSO oxidation and found that the synthesis worked well.PMID:30000929

Technical Brief 5′-Phosphorylation of RNA
The presence of a 5′ phosphate on RNA oligonucleotides is critical for a variety of biological functions. It is a required structural component for RIG-1 to recognize single-stranded viral RNA and turn on the innate immune response.1 Similarly, 5′-phosphorylation of siRNA and piRNA is also required for the RNAi machinery to properly load and suppress gene translation (in the case of double-stranded siRNA) and protect germline cells from genetic damage due to transposons (in the case of single-stranded piRNA).2 For more practical applications, 5′-phosphorylated RNA allows long RNA oligonucleotides to be synthesized through enzymatic ligation of shorter, more easily purified RNA oligonucleotides.3 The efficient production of pure, 5′-phosphorylated RNA oligonucleotides by chemical synthesis has been hampered by the inability to purify these oligonucleotides by reverse phase cartridges or HPLC. While there are a number of options for the DMT-On purification of DNA,4 they all require treatment with base following the removal of the DMT which leads to both strand scission and 2′-3′ migration of phosphodiester linkages within the RNA. However, a researcher at Horizon Discovery/Dharmacon, Dr. Shoeb Khan, approached us with an elegant solution to this problem using an existing product of ours – the photocleavable PC Spacer. He suggested that by coupling the PC Spacer to the 5′ terminus of the RNA during synthesis, the RNA oligo could be cleaved and deprotected while maintaining the 5′ trityl on the PC Spacer which then.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com

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