Material has interesting properties and can readily be stained with intercalators. The
Synthesis of symmetrically branched oligonucleotides with rigid core via two-stage solid-phase synthesis. synthesis of the rigid hybrids (Scheme 1) is more complicated than that of branched oligonucleotides with flexible chains, but it is still simpler than the solution phase syntheses of other symmetrical hybrids. 2 The combination of reversed or 5′-phosphoramidites (stage 1), onsupport phosphitylation, and conventional 3′-phosphoramidites ensures that all chains are attached via the 3′-terminus, but some off-synthesizer steps are required. Glen Research will be happy to make TPM available to interested researchers upon request. References:
1. M.Meng, et al., Chembiochem, 2009,10, 1335-9. 2. O.Plietzsch, et al., Org Biomol Chem, 2009,7,4734-43.
TEchNIcAL BRIEf – cROSSLINKING WITh cLIcK chEMISTRy
The click process is rapidly becoming the method of choice for key organic reactions useful in oligonucleotide modification.3184-13-2 custom synthesis 1 The advent of Copper (Cu1) catalyzed [3+2] azide-alkyne click chemistry (CuACC) has been the main reason for the surge in interest in this chemistry. CuACC reactions have simply improved the speed and performance of click chemistry. At the same time, the specificity of the reaction between an alkyne and an azide means that side reactions are very unlikely even in complex biological systems.21343-40-8 MedChemExpress One of our most common questions from researchers is how to create a specific crosslink between the strands of an oligonucleotide duplex.PMID:30137793 Most procedures use light to create a crosslink using a free radical mechanism. Photolytic crosslinking is typically low yielding with many side reactions possible. Figure 1 shows click chemistry being used 2 to form such an internucleotide crosslink. One DNA strand is simply modified using C8-Alkyne-dT (10-1540). The complementary strand is first modified using Amino-Modifier C6-dT (10-1039). A post-synthesis conjugation with Azidobutyrate NHS ester (50-1904) leads to the azido-modified strand. A subsequent CuACC click reaction leads to the intranucleotide crosslink in high yield. In a recent article, this crosslinking technique was extended to hairpin and hammerhead ribozymes.3 References:
1. A.H.El-Sagheer,andT.Brown,Chem Soc Rev, 2010,39,1388-405. 2. P.Kocalka,A.H.El-Sagheer,andT.Brown, Chembiochem, 2008,9,1280-1285. 3. A.H.El-Sagheer,andT.Brown,Proc Natl Acad Sci U S A,2010,107,15329-34.
GLEN-PAKTM PRODUcT UPDATE: NEW PROTOcOLS fOR PURIfIcATION
Glen-PakTM purification has become a very important part of routine oligonucleotide purification. Our customers challenge the Technical Support group to adapt Glen-Pak techniques to their purification needs. As always, we enjoy the challenge. In this article, we introduce a new cartridge designed for optimal purification of oligos produced on 200 nmole or lower scales in a high throughput environment. We have also developed procedures for purifying oligos containing 2′-OMe and/ or 2′-F RNA linkages. Finally, we describe Glen-Pak purification of disulfide containing oligonucleotides, followed by optional reduction with dithiothreitol and desalting on a second Glen-Pak cartridge. Step by step versions of these new protocols can be viewed in our latest update of the Glen-Pak User Guide found in the purification section of our website or by following this URL: http:// glenresearch/Technical/GlenPak_User_ Guide.pdf 50mg glen-PakTM dna cartrIdge for uP to 200 nmole Scale SyntHeSe.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
